What assay types does the engine support?

The assay engine supports in vitro biochemical assays (enzyme kinetics, IC50, KD), cell-based assays, and in vivo study evaluation. It covers competitive, noncompetitive, mixed, uncompetitive, and allosteric inhibition mechanisms.

Can the assay engine help with CRO selection?

Yes. The Study Evaluator module includes CRO outsourcing decision support, comparing 5 CRO partners across key dimensions including turnaround time, cost, and specialization.

What reports does the engine generate?

The Report Generator module produces scientific reports, CRO outsourcing briefs, and strategic decision memos formatted for internal team review, investor materials, or regulatory submission support.

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Bridge BioHealthConsulting LLC

Bridge BioHealth

Biochemical Assay &
Drug Discovery Engine

Design, evaluate, and interpret biochemical, cell-based, and in vivo studies with scientific rigor. Predict mechanisms of action. Profile compounds. Generate decision-ready reports.

Mechanism Types

12+

QC Validators

CRO Partners

Report Formats

Scientific Integrity · Confidentiality · Probabilistic Language

All conclusions use probabilistic framing. Compound structures are never disclosed. Internal identifiers only. Designed for real drug discovery decisions.

In VitroCell-BasedIn Vivo

Kinetics Analyzer — Km, Vmax, kcat, IC50, KD analysis with QC validation

Datasets

BBH-2347

Competitive

EGFR Kinase (WT) — Radiometric kinase assay

Batch: BTH-2026-047

2026-03-14

pH 7.425°Cn=3CV 7.7%

Kinetic Parameters

IC50

18.2 nM

9.8 nM

0.34 nM

12.4 µM

Vmax

8.7 nmol/min/mg

kcat

2.3 s⁻¹

Binding Kinetics (SPR)

kon

2.1e+6 M⁻¹s⁻¹

koff

7.1e-4 s⁻¹

kcat/Km (Efficiency)

185K M⁻¹s⁻¹

Efficient catalysis

Experimental Conditions

Buffer: 50 mM HEPES, 150 mM NaCl, 0.01% Tween-20

Cofactors: ATP (100 µM), MgCl₂ (10 mM)

IC50 shifted 3.2x with ATP concentration increase — consistent with competitive mechanism

Normal Hill Behavior

nH = 0.94. Hill coefficient consistent with simple 1:1 binding model.

Data Validation & QC

KD consistency

KD from koff/kon (0.34 nM) is consistent with reported KD. ✓

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Biochemical Assay &Drug Discovery Engine

BBH-2347

Biochemical Assay &
Drug Discovery Engine