Compare Datasets. Quantify Differences. Publish the Evidence.
Overlay two experimental datasets — wild-type vs mutant, treated vs control, batch A vs batch B — with automated fold-change analysis, confidence-scored deltas, and publication-ready comparison tables.
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Instrument: Cytiva Biacore T200
Model: 1:1 Langmuir binding with mass transport limitation
Instrument: Cytiva Biacore T200
Model: 1:1 Langmuir binding with mass transport limitation
Side-by-Side Parameter Table
8 parameters| Parameter | Wild-Type | 95% CI | Conf. | Gly246Val Mutant | 95% CI | Conf. |
|---|---|---|---|---|---|---|
| Kd | 12.4 | [10.1, 15.2] | 97% | 187.3 | [156.2, 224.5] | 93% |
| Kon | 2.80e+5 | [2.40e+5, 3.20e+5] | 95% | 8.40e+4 | [7.10e+4, 9.90e+4] | 91% |
| Koff | 3.50e-3 | [3.00e-3, 4.10e-3] | 96% | 0.02 | [0.01, 0.02] | 92% |
| Residence Time | 4.76 | [4.07, 5.56] | 95% | 1.06 | [0.91, 1.23] | 90% |
| Stoichiometry | 1.02 | [0.97, 1.07] | 98% | 0.94 | [0.88, 1] | 87% |
| ΔH | -8.20 | [-9.10, -7.30] | 94% | -4.10 | [-5.20, -3] | 88% |
| ΔS | 12.5 | [9.80, 15.2] | 89% | 28.3 | [22.1, 34.5] | 82% |
| ΔG | -12.1 | [-12.8, -11.4] | 95% | -12.5 | [-13.2, -11.8] | 90% |
Wild-Type — Model Quality
R²
0.9987
RMSE
0.0034
AIC
-2847.3
Model: 1:1 Langmuir binding with mass transport limitation
Instrument: Cytiva Biacore T200
Mass transport correction applied. Sensor surface regeneration verified across 50 cycles. Native kinase shows diffusion-limited association consistent with pre-organized ATP-binding pocket.
Gly246Val Mutant — Model Quality
R²
0.9952
RMSE
0.0071
AIC
-1983.4
Model: 1:1 Langmuir binding with mass transport limitation
Instrument: Cytiva Biacore T200
Gly246Val mutation introduces a bulky valine side chain at the gatekeeper position, sterically hindering compound entry into the ATP-binding cleft. Model fit quality reduced vs. WT (R² = 0.995 vs. 0.999), suggesting potential minor population of alternative binding modes.
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